A rep for De Niro declined to comment on the news.
The two, who married in 1997, have a 20-year-old son and a 6-year-old daughter.
The “Raging Bull” Oscar winner and Hightower had filed for divorce in 1999. The two began a contentious custody battle over their son, Elliott, the New York Daily News reported. But the two scrapped the legal proceedings and renewed their vows five years later.
De Niro was previously married to Diahnne Abbott. They are share two children as well. He also has two kids with ex-girlfriend Toukie Smith.
De Niro, 75, and Hightower, 63, met at a restaurant in London where Hightower worked as a hostess in the 1980s.