In a Thursday interview with KSDK-TV, Akin took a jab at Rove's decision to start the Conservative Victory Project, a new super PAC aimed at preventing far-right influences from overtaking establishment Republicans.
"Karl Rove has made himself an expert," Akin said. "I think I lost one race. He managed to lose about 12 of them in one night."
Akin's remarks arrive two months after he and Rove took respective shots at each other over the GOP's 2012 election struggles. Back in early February, Rove singled out Akin as part of the party's problems.
"It's amazing that people think Todd Akin was the best we could've come up with," Rove told Fox News. "He wasn't even a Tea Partier."
Akin fired back a week later, slamming Rove's project as a "disrespectful" endeavor that threatens the "grassroots heart of the party." When asked Thursday whether Republicans should reject Rove, Akin left that decision in the hands of the same grassroots forces.
"That is something that in a way is going to be settled by people who trust him with their money, or not," he told KSDK-TV.