The "Jeopardy!" champ lauded the 29-year-old Colts quarterback for retiring, but some Twitter users turned the comment right back at the game-show whiz.
Holzhauer donated $1,109.14 to a pancreatic cancer walk in the name of the venerable game show host, who has been fighting the disease.
"I knew I wasn't going to go on a long streak," Emma Boettcher said after falling in her third title defense.
Host Alex Trebek dubbed her "the giant killer" in her first match since she ended James Holzhauer's dominant run.
The 2004 record holder even jabbed at Holzhauer's stiff smile in a lighthearted exchange on Twitter.
The University of Chicago librarian defeated the gambler at his own game.
The man who had made his name with big, bold bets did something very unusual in Monday night's episode.
The "Jeopardy!" champ won 32 straight games and was just short of Ken Jennings' earnings record heading into Monday's episode.
A few viewers celebrated that they knew something the record-breaking champ didn't.
The gambling disclosure spiced up a routine 30th consecutive victory as Holzhauer neared Ken Jennings' earnings record.
Nate Scheffey refused to be intimidated in a tight match that came down to Final Jeopardy.
The "Jeopardy!" champ wagered "two dimes."
The looks from the other contestants during his 24th-straight victory were priceless.
The Patriots quarterback made a promise if the game-show champ lost .
"We have to get this guy in baseball," one team executive said.
The game show host also told "Good Morning America" that Holzhauer's run has changed his perception about all-time champ Ken Jennings' record.
The professional sports gambler nailed 41 out of 41 responses to shatter his single-game earnings mark.