The U.S. Fish and Wildlife Service and an environmental organization offered money in a bid to crack the tough case.
The Lassen Pack is now up to at least seven, according to state wildlife officials.
“We’re going to fight this in any way possible," said Jamie Clark of Defenders of Wildlife.
The Estonian canine was stuck in a maze of ice when construction workers saved him.
The wild wolf, also known as 926F, died the same way her famous mother did in 2012.
Around 5,000 wolves currently live in the lower 48 states.
Wolf tourism is bringing more benefits than hunting.
“We have no idea what this was until we get a DNA report back."
Conservationists say the decision could help save other imperiled wildlife.
The Endangered Species Act of 1973 protects over a thousand species of plants and animals. However, its own future hangs in the balance as the House Committee on Natural Resources hears from bills that aim to weaken its power.