"When you've got something to say, you've got to speak up, all right," said Gaga before performing the queer anthem at her Las Vegas residency.
The legendary singer died on July 21 at the age of 96.
Over one week after the singer's death, Gaga remembered her friend and frequent collaborator in a moving tribute.
“I hope you know this time to myself has been extremely healing and recharging for my heart, mind, body, and creativity,” wrote Gaga.
The advisory group was reestablished in September after its previous members resigned en masse under former President Donald Trump.
In contrast to her 2019 formal duet with Bradley Cooper, Gaga opted for a minimalist approach when taking the stage this year.
The "Poker Face" singer reacted to commotion behind her at the Academy Awards.
Jennifer McBride was sentenced to two years probation after she was arrested in connection with stealing the singer's French bulldogs in 2021.
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The gunman, who also stole the French bulldogs, has taken a plea deal.